(2018) Radioprotective effect of melatonin on expression of Cdkn1a and Rad50 genes in rat peripheral blood. Journal of Cancer Research and Therapeutics. S1070-S1075. ISSN 0973-1482
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Abstract
Objective: Ionizing radiation is a critical threat to biomolecules, especially DNA. Various combinatorial compounds have been studied to protect this biomolecule. Melatonin has been reported as a direct and indirect free radical scavenger, but in this study, we explored the effect of melatonin on assisting in DNA repair by expression of Cdkn1a and Rad50; both of these genes are involved in DNA repair signaling, induced by radiation in rat peripheral blood. Materials and Methods: Rats were irradiated with single whole-body linear accelerator X-ray radiation doses of 2 and 8 Gy with or without melatonin (100 mg/kg body weight) pretreatments. The rats were randomly divided into nine groups and given an intraperitoneal injection of melatonin or the same volume of vehicle alone 1 h before radiation. Blood samples were taken 8, 24, and 48 h postradiation to measure gene expression of Cdkn1a and Rad50 using quantitative reverse transcription polymerase chain reaction technique. Results: Melatonin pretreatment increased the expression of Cdkn1a and Rad50 in 8 and 24 h postradiations (2 and 8 Gy) (P < 0.05), and there was no significant difference in 48 h postradiation compared to the radiation-only and vehicle plus radiation (2 and 8 Gy) groups. Conclusions: Based on our results, pretreatment with melatonin (100 mg/kg) may ameliorates injurious effects of 2 and 8 Gy ionization radiation by increasing the expression level of Cdkn1a and Rad50 in rat peripheral blood and assist in DNA double-strand breaks repair, especially during the early postradiation.
Item Type: | Article |
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Keywords: | DNA repair melatonin peripheral blood quantitative reverse transcription polymerase chain reaction X-ray radiation strand break repair induced oxidative damage gamma-irradiation radiation lymphocytes amifostine radicals phase model Oncology |
Divisions: | |
Page Range: | S1070-S1075 |
Journal or Publication Title: | Journal of Cancer Research and Therapeutics |
Volume: | 14 |
Identification Number: | 10.4103/0973-1482.196758 |
ISSN: | 0973-1482 |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/3777 |
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