Abstract

Di- and poly-nuclear Manganese-Cobalt complexes (dnc and pnc) were selected to be investigated in their anticancer activities against MCF-7 breast cancer cell lines. The results show that the pnc can reduce the cell viability to about 12 of the control after 72 h of exposure, in the event that dnc don't have a significant cellular cytotoxic impact on cell viability in MCF-7 cell line. The interaction of complexes with beta LG and HSA were investigated and showed that (i) both complexes strongly quenched the fluorescence of proteins through the static quenching (ii) in the dnc-protein systems, HSA had stronger binding affinity toward complex than KG, (iii) the association affinity of pnc bound to HSA or beta LG were close to each other; (iv) the interaction of pnc with two proteins was greater than that of dnc (v) hydrogen bonding and hydrophobic interactions (especially in the pnc) are predominant forces in the binding process (vi) the binding of pnc and dnc to HSA and beta LG can induce conformational changes of these proteins. These results can confirm that in addition to the type of ligands, the nuclear numbers and surface of the metal complex has a major impact on its biological activity. (C) 2 018 Elsevier B.V. All rights reserved.