(2013) Targeted fluoromagnetic nanoparticles for imaging of breast cancer MCF-7 cells. Advanced Pharmaceutical Bulletin. pp. 189-195. ISSN 22285881 (ISSN)
Full text not available from this repository.
Abstract
Purpose: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. Methods: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. Results: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. Conclusion: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR. © 2013 by Tabriz University of Medical Sciences.
| Item Type: | Article |
|---|---|
| Keywords: | Breast cancer Folate receptor Internalization Magnetic nanoparticles MCF-7 cells macrogol magnetic nanoparticle magnetite dopamine hydrobromide polyethylene glycol fluorescein isothiocyanate nanoconjugate magnetite dopamine hydrobromide polyethylene glycol folic acid nanoconjugate unclassified drug article cell interaction cell strain MCF 7 conjugation decomposition flow cytometry fluorescence microscopy human human cell image analysis infrared spectroscopy nanoimaging scanning electron microscopy transmission electron microscopy |
| Divisions: | |
| Page Range: | pp. 189-195 |
| Journal or Publication Title: | Advanced Pharmaceutical Bulletin |
| Volume: | 3 |
| Number: | 1 |
| Identification Number: | 10.5681/apb.2013.031 10.3109/1061186X.2012.750325; Hu, F.X., Neoh, K.G., Kang, E.T., Synthesis and in vitro anti-cancer evaluation of tamoxifen-loaded magnetite/plla composite nanoparticles (2006) Biomaterials, 27 (33), pp. 5725-5733; Akça, Ö., Ünak, P., |
| ISSN: | 22285881 (ISSN) |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.zbmu.ac.ir/id/eprint/3275 |
Actions (login required)
 |
View Item |