(2014) Cytoprotective effects of hydrophilic and lipophilic extracts of Pistacia vera against oxidative versus carbonyl stress in rat hepatocytes. Iranian Journal of Pharmaceutical Research. pp. 1263-1277. ISSN 17350328 (ISSN)
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Abstract
This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation, mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to prepare crude pistachios extracts, which were then used to screen for in-vitro cytoprotection of freshly isolated rat hepatocytes against these toxins. The order of protection by Pistacia vera extracts against both hydroperoxide induced oxidative stress (ROS formation) and glyoxal induced protein carbonylation was: pistachio methanolic extract > pistachio water extract, gallic acid, catechin > α-tochoferol and pistachio ethyl acetate extract. Finally due to higher protection achieved by methanolic extract even compared to sole pretreatment of gallic acid, catechin or α-tochoferol, we suggest that cytoprotection depends on the variety of polar and non-polar compounds found in methanolic extract, it is likely that multiple cytoprotective mechanisms are acting against oxidative and carbonyl induced cytotoxicity. To our knowledge, we are the first to report the cytoprotective activity of Pistacia vera extracts against oxidative and carbonyl stress seen in type 2 diabetes hepatocytes model. © 2014 by School of Pharmacy.
Item Type: | Article |
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Keywords: | Cytotoprotection Lysosomes Mitochondria Oxidative stress Pistacia vera Protein carbonylation alpha tocopherol carbonyl derivative catechin gallic acid glutathione glutathione disulfide penicillamine Pistacia vera extract plant extract reactive oxygen metabolite unclassified drug animal cell Article cell protection cell viability controlled study cytotoxicity diabetes mellitus lipid peroxidation liver cell male membrane damage mitochondrial membrane potential nonhuman pistachio rat spectrophotometry |
Divisions: | |
Page Range: | pp. 1263-1277 |
Journal or Publication Title: | Iranian Journal of Pharmaceutical Research |
Volume: | 13 |
Number: | 4 |
ISSN: | 17350328 (ISSN) |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/3252 |
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