(2017) Modulation of radiation-induced base excision repair pathway gene expression by melatonin. Journal of Medical Physics. pp. 245-250. ISSN 09716203 (ISSN)
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Abstract
Objective: Approximately 70 of all cancer patients receive radiotherapy. Although radiotherapy is effective in killing cancer cells, it has adverse effects on normal cells as well. Melatonin (MLT) as a potent antioxidant and anti-inflammatory agent has been proposed to stimulate DNA repair capacity. We investigated the capability of MLT in the modification of radiation-induced DNA damage in rat peripheral blood cells. Materials and Methods: In this experimental study, male rats (n = 162) were divided into 27 groups (n = 6 in each group) including: irradiation only, vehicle only, vehicle with irradiation, 100 mg/kg MLT alone, 100 mg/kg MLT plus irradiation in 3 different time points, and control. Subsequently, they were irradiated with a single whole-body X-ray radiation dose of 2 and 8 Gy at a dose rate of 200 MU/min. Rats were given an intraperitoneal injection of MLT or the same volume of vehicle alone 1 h prior to irradiation. Blood samples were also taken 8, 24, and 48 h postirradiation, in order to measure the 8-oxoguanine glycosylase1 (Ogg1), Apex1, and Xrcc1 expression using quantitative real-time-polymerase chain reaction. Results: Exposing to the ionizing radiation resulted in downregulation of Ogg1, Apex1, and Xrcc1 gene expression. The most obvious suppression was observed in 8 h after exposure. Pretreatments with MLT were able to upregulate these genes when compared to the irradiation-only and vehicle plus irradiation groups (P < 0.05) in all time points. Conclusion: Our results suggested that MLT in mentioned dose may result in modulation of Ogg1, Apex1, and Xrcc1 gene expression in peripheral blood cells to reduce X-ray irradiation-induced DNA damage. Therefore, administration of MLT may increase the normal tissue tolerance to radiation through enhancing the cell DNA repair capacity. We believed that MLT could play a radiation toxicity reduction role in patients who have undergone radiation treatment as a part of cancer radiotherapy. © 2017 Journal of Medical Physics.
Item Type: | Article |
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Keywords: | Base excision repair gene expression melatonin radiation real-time-polymerase chain reaction XRCC1 protein animal cell animal experiment Apex1 gene Article cell protection controlled study DNA damage DNA damage response down regulation excision repair gene gene expression regulation gene repression ionizing radiation leukocyte lymphocyte male nonhuman Ogg1 gene radiation dose radiation exposure radiation protection radiation tolerance radiotherapy rat real time polymerase chain reaction upregulation Wistar rat X ray Xrcc1 gene |
Divisions: | |
Page Range: | pp. 245-250 |
Journal or Publication Title: | Journal of Medical Physics |
Volume: | 42 |
Number: | 4 |
Identification Number: | 10.4103/jmp.JMP₉₁₇ |
ISSN: | 09716203 (ISSN) |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/3027 |
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