(2013) Targeted fluoromagnetic nanoparticles for imaging of breast cancer mcf-7 cells. Advanced Pharmaceutical Bulletin. pp. 189-95. ISSN 2228-5881 (Print) 2228-5881 (Linking)
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Abstract
PURPOSE: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. METHODS: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. RESULTS: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. CONCLUSION: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR.
Item Type: | Article |
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Keywords: | Breast cancer Folate receptor Internalization MCF-7 cells Magnetic nanoparticles |
Divisions: | |
Page Range: | pp. 189-95 |
Journal or Publication Title: | Advanced Pharmaceutical Bulletin |
Volume: | 3 |
Number: | 1 |
Identification Number: | 10.5681/apb.2013.031 |
ISSN: | 2228-5881 (Print) 2228-5881 (Linking) |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/2963 |
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