(2013) The role of nitric oxide in the PKA inhibitor induced spatial memory deficits in rat: Involvement of choline acetyltransferase. European Journal of Pharmacology. pp. 478-485. ISSN 0014-2999
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Abstract
Several lines of evidence show that cAMP-PKA signaling pathway plays critical role in memory functions and suggest nitric oxide as an important modulator in learning and memory. In this study, we assessed the effects of intra-hippocampal infusion of H-89, a selective PKAII inhibitor, and 1400 W, a selective inducible nitric oxide synthase (iNOS) inhibitor, on spatial memory in rats. By using the Morris water maze, spatial memory retention parameters were examined 48 h after the infusions through measuring escape latency, traveled distance, and swimming speed. The rats receiving intra-hippocampal infusions of 1400 W (100 mu M/side) showed a significant reduction (*P < 0.05) in escape latency and traveled distance in comparison with the control saline group. In contrast, a significant increase (**P < 0.01) in escape latency and traveled distance was observed after infusion of 10 mu M H-89. Moreover, among combination groups, co-administration of 1400 W (400 mu M/side) with 10 mu M/side of H-89 caused a significant reduction (*P < 0.05) in escape latency and traveled distance in comparison with the H-89 group. Also, we evaluated the molecular effects of 1400 W on the expression of choline acetyltransferase (ChAT), a cholinergic marker, in the CA1 region of the hippocampus and medial septal area (MSA). Immunohistochemical analysis of post training bilateral intra-hippocampal infusion of 1400 W revealed a significant increase in ChAT immunoreactivity levels in both the CA1 and the MSA regions. Overall, the results suggest that 1400 W has protective effect against H89-induced spatial memory impairment. Moreover, the observed memory improvements caused by 1400 W infusions, might be due to interaction of iNOS with the cholinergic system. (c) 2013 Elsevier B.V. All rights reserved.
Item Type: | Article |
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Keywords: | inducible nitric oxide synthase spatial memory morris water maze protein kinase a long-term potentiation morris water maze vesicular acetylcholine transporter nicotine-bucladesine combination methyl-d-aspartate glucose deprivation kappa-b intrahippocampal infusion transcription factors synaptic plasticity |
Divisions: | |
Page Range: | pp. 478-485 |
Journal or Publication Title: | European Journal of Pharmacology |
Volume: | 714 |
Number: | 1-3 |
Identification Number: | 10.1016/j.ejphar.2013.06.039 |
ISSN: | 0014-2999 |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/2747 |
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