(2014) Ultrasensitive detection of lead (II) based on fluorescent aptamer-functionalized carbon nanotubes. Environmental Toxicology and Pharmacology. pp. 1236-1242. ISSN 1382-6689
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Abstract
Lead contamination is a serious environmental problem with toxic effects in human. Here, we developed a simple and sensitive sensing method employing ATTO 647N/aptamer-SWNT ensemble for detection of Pb2+. This method is based on the super quenching capability of single-walled carbon nanotubes (SWNTs), high affinity of the aptamer toward Pb2+ and different propensities of ATTO 647N-aptamer and ATTO 647N-aptamer/Pb2+ complex for adsorption on SWNTs. In the absence of Pb2+, the fluorescence of ATTO 647N-aptamer is efficiently quenched by SWNTs. Upon addition of Pb2+, the aptamer binds to its target, leading to the formation of a G-quadruplex/Pb2+ complex and does not interact with SWNTs and ATTO 647N-aptamer starts fluorescing. This sensor exhibited a high selectivity toward Pb2+ and a limit of detection (LOD) as low as 0.42 nM was obtained. Also this sensor could be applied for detection of Pb2+ ions in tap water and biological sample like serum with high sensitivity. (C) 2014 Elsevier B.V. All rights reserved.
Item Type: | Article |
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Keywords: | atto 647n aptamer sensor lead swnt highly sensitive detection energy-transfer graphene oxide toxicity amplification biosensor delivery sensor green ions |
Divisions: | |
Page Range: | pp. 1236-1242 |
Journal or Publication Title: | Environmental Toxicology and Pharmacology |
Volume: | 37 |
Number: | 3 |
Identification Number: | 10.1016/j.etap.2014.04.020 |
ISSN: | 1382-6689 |
Depositing User: | مهندس مهدی شریفی |
URI: | http://eprints.zbmu.ac.ir/id/eprint/2727 |
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