(2014) Fe3O4 nanoparticles engineered for plasmid DNA delivery to Escherichia coli. Journal of Nanoparticle Research. ISSN 1388-0764
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Abstract
Heat shock treatment is the most popular method for transformation of Escherichia coli. We have used 19-nm Fe3O4 nanoparticles for improving heat shock protocol. PGEM-T (3,000 bp) and pCAMBIA (8,428 bp) were used as test plasmids for transformation of competent E. coli cells (strains DH5 alpha and Jm107) obtained from heat shock- and CaCl2-treated bacteria. A combination of heat shock and Fe3O4 nanoparticles led to a significant increase (6-10 fold) in number of transformed colonies in comparison with heat shock alone. The percent increase in transformation efficiency was higher for larger pCAMBIA plasmids compared to PGEM-T. The transformation efficiency decreased in the absence of CaCl2 and increased by addition of glycerol to the bacterial culture.
| Item Type: | Article |
|---|---|
| Keywords: | e. coli transformation pcambia pgem-t dh5 alpha jm107 bacteria molecular biology iron-oxide nanoparticles magnetic nanoparticles beta-escin transformation glycerol cells membrane proteins molecules bacteria |
| Divisions: | |
| Journal or Publication Title: | Journal of Nanoparticle Research |
| Volume: | 16 |
| Number: | 8 |
| Identification Number: | Artn 2521 10.1007/S11051-014-2521-0 |
| ISSN: | 1388-0764 |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.zbmu.ac.ir/id/eprint/2719 |
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