Abstract

In this study, the karyotype, morphology and anatomy of the petiole and stem the herb Salvia nemorosa was discussed. After collecting and identifying plant, it conducted qualitative and quantitative traits, then, Very thin slices prepared and the samples were stained. To prepare a karyotype, three methods were used which method use of colchicine was more effective. S. nemorosa covered with soft short glandular trichome with low-density and dense non glandular trichome. The general shape of petiole, crescent-shaped, 5-bundle sheath of the center and 2 bundle sheath on either side there. Circular stem epidermis cells, Pith parenchyma cell with 40 to 50 layers of cells polygonal, karyological results showed that the chromosome number of S. nemorosa (2n = 14) with karyotype formula is 4 sm + 3m.